Flow cytometry analysis overlay peak curve showing U87 cells stained with SLN monoclonal antibody (Product # MA5-50293) (red line) at 1:60. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1 µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1 µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.